Occurrence of erythromycin residues in sheep milk. Validation of an analytical method

García Mayor, M. Asunción, Paniagua González, Gema, Soledad-Rodríguez, B.E., Garcinuño Martínez, Rosa María, Fernández Hernando, Pilar y Durand-Alegría, J.S. . (2015) Occurrence of erythromycin residues in sheep milk. Validation of an analytical method. Food and Chemical Toxicology. 2015, 78, 22-36.

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Título Occurrence of erythromycin residues in sheep milk. Validation of an analytical method
Autor(es) García Mayor, M. Asunción
Paniagua González, Gema
Soledad-Rodríguez, B.E.
Garcinuño Martínez, Rosa María
Fernández Hernando, Pilar
Durand-Alegría, J.S.
Materia(s) Ciencias
Abstract The paper describes a new and selective analytical sample treatment for quantitative extraction and preconcentration of erythromycin in presence of other macrolide antibiotics in sheep milk samples. The methodology is based on the use of a molecular imprinted polymer (MIP) employed as solid phase extraction sorbent (MISPE). The synthesized material by bulk polymerization using erythromycin (ERY) as template was evaluated as solid phase extraction sorbent, in a novel sample treatment technique that can be coupled to high-performance liquid chromatography with diode-array detector (HPLC-DAD). MIP selectivity was studied for other macrolide antibiotics with similar structures, such as tylosin (TYL), spiramycin (SPI), josamycin (JOS), roxithromycin (ROX) and ivermectin (IVER) getting recoveries for these interferents lower than 35%, for all cases except for ROX, which recoveries were around 85%. The variables affecting the molecularly imprinted solid-phase extraction (MISPE) procedure were optimized to select the best conditions of selectivity and sensitivity to determine ERY at concentration levels established by EU legislation in sheep milk. Under the selected experimental conditions, quantification limit was 24.1 µg kg−1. Recoveries were higher than 98%, with RSDs between 0.7% and 2%. The proposed MISPE-HPLC method was validated and successfully applied to ERY analysis in sheep milk samples.
Palabras clave Erythromycin
Molecularly imprinted solid-phase extraction
Sheep milk
HPLC-DAD
Editor(es) Elsevier
Fecha 2015-04
Formato application/pdf
Identificador bibliuned:DptoCA-FCIE-Articulos-Gpaniagua-0017
http://e-spacio.uned.es/fez/view/bibliuned:DptoCA-FCIE-Articulos-Gpaniagua-0017
DOI - identifier https://doi.org/10.1016/j.fct.2014.12.020
ISSN - identifier 0278-6915 - eISSN: 1873-6351
Nombre de la revista Food and Chemical Toxicology
Número de Volumen 78
Página inicial 22
Página final 36
Publicado en la Revista Food and Chemical Toxicology. 2015, 78, 22-36.
Idioma eng
Versión de la publicación acceptedVersion
Tipo de recurso Article
Derechos de acceso y licencia http://creativecommons.org/licenses/by-nc-nd/4.0
info:eu-repo/semantics/openAccess
Tipo de acceso Acceso abierto
Notas adicionales The registered version of this article, first published in Food and Chemical Toxicology, is available online at the publisher's website: Elsevier, https://doi.org/10.1016/j.fct.2014.12.020
Notas adicionales La versión registrada de este artículo, publicado por primera vez en Food and Chemical Toxicology, está disponible en línea en el sitio web del editor: Elsevier, https://doi.org/10.1016/j.fct.2014.12.020

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Creado: Wed, 20 Mar 2024, 04:39:39 CET