Molecularly imprinted SPE and MEKC with in-capillary sample preconcentration for the determination of digoxin in human urine.

Guijarro-Díez, Miguel, Paniagua González, Gema, Fernández Hernando, Pilar, Crego, Antonio Luis y Marina, María Luisa . (2012) Molecularly imprinted SPE and MEKC with in-capillary sample preconcentration for the determination of digoxin in human urine.. Electrophoresis. 2012, 33(11), 1852-1588.

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Título Molecularly imprinted SPE and MEKC with in-capillary sample preconcentration for the determination of digoxin in human urine.
Autor(es) Guijarro-Díez, Miguel
Paniagua González, Gema
Fernández Hernando, Pilar
Crego, Antonio Luis
Marina, María Luisa
Materia(s) Ciencias
Abstract Molecularly imprinted solid-phase extraction (MISPE) combined with MEKC was used for clean-up, preconcentration and determination of digoxin in the presence of its aglycon digoxin (digoxigenin) in human urine samples. In addition, the use of an in-capillary sample concentration electrophoretic technique by sweeping was investigated to enhance the concentration sensitivity in MEKC. The highly selective, fast and effective sample pretreatment by MISPE along with the preconcentration by sweeping could overcome the low sensitivity of the highly efficient capillary electrophoresis separation with UV detection. The optimization of the variables affecting the separation as well as MISPE conditions procedure was carried out to select the best conditions of selectivity and sensitivity to determine digoxin at low concentration levels in urine. To demonstrate the suitability of the developed method several analytical characteristics (selectivity, linearity, accuracy, precision, and LOD) were evaluated. Satisfactory results were obtained in terms of linearity (r > 0.99), recovery (95.4–96.5% with RSD from 1.3% to 2.6%), precision (RSD from 0.3% to 1.7% for migration times and from 2.1% to 7.3% for corrected peak areas), and sensitivity (LODs of 6 μg/L with 5 mL of sample or 1.2 μg/L with 25 mL). The proposed MISPE-MEKC method was satisfactorily applied to the analysis of spiked human urine samples achieving a concentration factor up to 7500-fold.
Palabras clave Digoxin
In-capillary sample preconcentration
Micellar electrokinetic chromatography
Molecularly imprinted solid-phase extraction
Sweeping
Editor(es) Wiley
Fecha 2012-06
Formato application/pdf
Identificador bibliuned:DptoCA-FCIE-Articulos-Gpaniagua-0015
http://e-spacio.uned.es/fez/view/bibliuned:DptoCA-FCIE-Articulos-Gpaniagua-0015
DOI - identifier https://doi.org/10.1002/elps.201100588
ISSN - identifier 0173-0835 - eISSN 1522-2683
Nombre de la revista Electrophoresis
Número de Volumen 33
Número de Issue 11
Página inicial 1852
Página final 1588
Publicado en la Revista Electrophoresis. 2012, 33(11), 1852-1588.
Idioma eng
Versión de la publicación acceptedVersion
Tipo de recurso Article
Derechos de acceso y licencia http://creativecommons.org/licenses/by-nc-nd/4.0
info:eu-repo/semantics/openAccess
Tipo de acceso Acceso abierto
Notas adicionales The registered version of this article, first published in Electrophoresis, is available online at the publisher's website: Wiley, https://doi.org/10.1002/elps.201100588
Notas adicionales La versión registrada de este artículo, publicado por primera vez en Electrophoresis, está disponible en línea en el sitio web del editor: Wiley, https://doi.org/10.1002/elps.201100588

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Creado: Tue, 19 Mar 2024, 05:58:58 CET