Selective extraction of diazepam and its metabolites from urine samples by a molecularly imprinted solid phase extraction (MISPE) method

Gil Tejedor, Ana María, Bravo Yagüe, Juan Carlos, Paniagua González, Gema, Garcinuño Martínez, Rosa María y Fernández Hernando, Pilar . (2024) Selective extraction of diazepam and its metabolites from urine samples by a molecularly imprinted solid phase extraction (MISPE) method. Polymers, 2024, 16, 635

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Título Selective extraction of diazepam and its metabolites from urine samples by a molecularly imprinted solid phase extraction (MISPE) method
Autor(es) Gil Tejedor, Ana María
Bravo Yagüe, Juan Carlos
Paniagua González, Gema
Garcinuño Martínez, Rosa María
Fernández Hernando, Pilar
Materia(s) Ciencias
Abstract In this research, a molecularly imprinted polymer (MIP) was synthesized by precipitation polymerization using oxazepam (OZ) as a template molecule and was subsequently applied as a selective sorbent for the extraction of diazepam (DZP) and its metabolites in urine samples using an SPE cartridge. OZ, temazepam (TZ), nordiazepam (NZ) and DZP were analyzed in the final extracts by high-performance liquid chromatography with diode array detection (HPLC-DAD). The SPE extraction steps were optimized, and the evaluation of an imprinting factor was carried out. The selectivity of the method for OZ versus structurally related benzodiazepines (BZDs), such as bromazepam (BRZ), tetrazepam (TTZ) and halazepam (HZ), was investigated. Under the optimum conditions, the proposed methodology provided good linearity in the range of 10–1500 ng/mL, with limit of detection values between 13.5 and 21.1 ng/mL and recovery levels for DZP and its metabolites from 89.0 to 93.9% (RSD ≤ 8%) at a concentration level of 1000 ng/mL. The proposed method exhibited good selectivity, precision and accuracy and was applied to the analysis of urine samples from a real case of DZP intake.
Palabras clave benzodiazepines
molecular imprinting solid-phase extraction
urine samples
HPLC-DAD
Editor(es) MDPI
Fecha 2024-02
Formato application/pdf
Identificador bibliuned:DptoCA-FCIE-Articulos-Gpaniagua-0013
http://e-spacio.uned.es/fez/view/bibliuned:DptoCA-FCIE-Articulos-Gpaniagua-0013
DOI - identifier https://doi.org/10.3390/polym16050635
ISSN - identifier 2073-4360
Nombre de la revista Polymers
Número de Volumen 16
Número de Issue 5
Publicado en la Revista Polymers, 2024, 16, 635
Idioma eng
Versión de la publicación publishedVersion
Tipo de recurso Article
Derechos de acceso y licencia http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
Tipo de acceso Acceso abierto
Notas adicionales The registered version of this article, first published in Polymers, is available online at the publisher's website: MDPI, https://doi.org/10.3390/polym16050635
Notas adicionales La versión registrada de este artículo, publicado por primera vez en Polymers, está disponible en línea en el sitio web del editor: MDPI, https://doi.org/10.3390/polym16050635

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Creado: Tue, 19 Mar 2024, 03:49:26 CET