A MIP-based flow-through fluoroimmunosensor as an alternative to immunosensors for the determination of digoxin serum samples

Paniagua González, Gema, Fernández Hernando, Pilar y Durand Alegría, J. S. . (2009) A MIP-based flow-through fluoroimmunosensor as an alternative to immunosensors for the determination of digoxin serum samples. Analytical and Bioanalytical Chemistry 394 (2009), 963-970.

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Título A MIP-based flow-through fluoroimmunosensor as an alternative to immunosensors for the determination of digoxin serum samples
Autor(es) Paniagua González, Gema
Fernández Hernando, Pilar
Durand Alegría, J. S.
Materia(s) Ciencias
Abstract This work reports a comparative study of two automated flow-through fluorosensors for the determination of digoxin in serum samples: an immunosensor with an anti-digoxin polyclonal antibody as the reactive phase permanently immobilized on CPG, and a sensor with a selective reaction system based on a methacrylic molecularly imprinted polymer (MIP) synthesized by bulk polymerisation. The variables affecting the sensitivity and dynamic range of the sensors (e.g., the carrier and elution solutions, flow-rates, pH and reagent concentrations) were optimised and the binding characteristics of their reactive phases compared in a competitive fluorescent assay. Digoxin was reproducibly determined by both sensors at the mg L-1 level (detection limit= 1.20×10-3 mg L-1 and RSD= 4-7% for the immunosensor; detection limit= 1.7×10-5 mg L-1 and RSD= 1-2% for the MIP sensor). No cross reactivity with digoxinrelated compounds was seen for either sensor at a digoxin:interferent ratio of 1:100. The lifetime of the immunosensor was about 50 immunoassays; its shelf life, when unused, is about three months. The lifetime of the MIP sensor was over 18 months. Both sensors were used to determine the digoxin concentration of human serum samples with satisfactory results.
Palabras clave Digoxin
Permanent immobilization
Molecular imprinting
Flow-through fluorosensor
Serum analysis
Editor(es) Springer
Fecha 2009-01-09
Formato application/pdf
Identificador bibliuned:DptoCA-FCIE-Articulos-Gpaniagua-0011
http://e-spacio.uned.es/fez/view/bibliuned:DptoCA-FCIE-Articulos-Gpaniagua-0011
DOI - identifier https://doi.org/10.1007/s00216-008-2584-9
ISSN - identifier 1618-2642 - eISSN 1618-2650
Nombre de la revista Analytical and Bioanalytical Chemistry
Número de Volumen 394
Página inicial 963
Página final 970
Publicado en la Revista Analytical and Bioanalytical Chemistry 394 (2009), 963-970.
Idioma eng
Versión de la publicación acceptedVersion
Tipo de recurso Article
Derechos de acceso y licencia http://creativecommons.org/licenses/by-nc-nd/4.0
info:eu-repo/semantics/openAccess
Tipo de acceso Acceso abierto
Notas adicionales The registered version of this article, first published in Analytical and Bioanalytical Chemistry, is available online at the publisher's website: Springer, https://doi.org/10.1007/s00216-008-2584-9
Notas adicionales La versión registrada de este artículo, publicado por primera vez en Analytical and Bioanalytical Chemistry, está disponible en línea en el sitio web del editor: Springer, https://doi.org/10.1007/s00216-008-2584-9

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Creado: Wed, 13 Mar 2024, 05:39:05 CET