Permanent oriented antibody immobilization for digoxin determination with a flow-through fluoroimmunosensor

Fernández Hernando, Pilar, Durand Alegría, J.S., Pérez-Conde, C. y Paniagua González, Gema . (2003) Permanent oriented antibody immobilization for digoxin determination with a flow-through fluoroimmunosensor. Analytical and Bioanalytical Chemistry 375 (2003), 1020-1023.

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Título Permanent oriented antibody immobilization for digoxin determination with a flow-through fluoroimmunosensor
Autor(es) Fernández Hernando, Pilar
Durand Alegría, J.S.
Pérez-Conde, C.
Paniagua González, Gema
Materia(s) Ciencias
Abstract Digoxin is a very important compound in clinical chemistry and is indicated in the treatment of congestive heart failure and artery disease. The measurement of serum digoxin concentration is necessary owing to the narrow therapeutic range of this drug. Further, even with similar dosage regimens, the biological response of patients often results in very different concentrations of digoxin in serum. Concentrations of greater than 2.6 mmol/L are generally interpreted as toxic in adult patients. Most methods for digoxin determination are based on gas chromatography or radiochemical and enzymatic immunoassay techniques. However, some of these methods are tedious and difficult to automate. Nowadays, they are being replaced by more practical immunoassay techniques, involving, for example, fluorescent immunosensors that allow rapid, automated and selective digoxin determinations. This paper reports a new flow–through fluoroimmunosensor for digoxin determination, the function of which is based on antibodies immobilized on an inmunoreactor of controlled pore glass (CPG). The immunosensor has a detection limit of 1.20 μg/L and provides high reproducibility (RSD = 4.5% for a concentration of 0.0025 mg/L, and RSD = 6.7% for 0.01 mg/L). The optimum working concentration range was found to be 1.2×10-3 - 4.0×10-2 mg/L. The lifetime of the immunosensor was about 50 immunoassays, if stored unused its lifetime can be extended to three months. A sample speed of about 10–12 samples per hour can be attained. Possible interference from substances with structures similar to digoxin (morphine, heroine, tebaine, codeine, pentazocine and narcotine) was investigated. No cross-reactivity was seen at the highest digoxin:interferent ratio studied (1:100). The proposed fluoroimmunosensor was successfully used to determine digoxin concentrations in human serum samples.
Palabras clave flow-through fluoroimmunosensor
digoxin
permanent immobilization
serum samples
Editor(es) Springer
Fecha 2003-02-28
Formato application/pdf
Identificador bibliuned:DptoCA-FCIE-Articulos-Gpaniagua-0010
http://e-spacio.uned.es/fez/view/bibliuned:DptoCA-FCIE-Articulos-Gpaniagua-0010
DOI - identifier https://doi.org/10.1007/s00216-002-1746-4
ISSN - identifier 1618-2642 - eISSN 1618-2650
Nombre de la revista Analytical and Bioanalytical Chemistry
Número de Volumen 375
Página inicial 1020
Página final 1023
Publicado en la Revista Analytical and Bioanalytical Chemistry 375 (2003), 1020-1023.
Idioma eng
Versión de la publicación acceptedVersion
Tipo de recurso Article
Derechos de acceso y licencia http://creativecommons.org/licenses/by-nc-nd/4.0
info:eu-repo/semantics/openAccess
Tipo de acceso Acceso abierto
Notas adicionales The registered version of this article, first published in Analytical and Bioanalytical Chemistry, is available online at the publisher's website: Springer, https://doi.org/10.1007/s00216-002-1746-4
Notas adicionales La versión registrada de este artículo, publicado por primera vez en Analytical and Bioanalytical Chemistry, está disponible en línea en el sitio web del editor: Springer, https://doi.org/10.1007/s00216-002-1746-4

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Creado: Wed, 13 Mar 2024, 05:14:41 CET