Determination of digoxin in serum samples using a flow-through fluorosensor based on a molecularly imprinted polymer

Paniagua González, Gema, Fernández Hernando, Pilar y Durand Alegría, J. S. . (2008) Determination of digoxin in serum samples using a flow-through fluorosensor based on a molecularly imprinted polymer. Biosensors and Bioelectronics. 2008. 23 (11), 1754-1758

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Título Determination of digoxin in serum samples using a flow-through fluorosensor based on a molecularly imprinted polymer
Autor(es) Paniagua González, Gema
Fernández Hernando, Pilar
Durand Alegría, J. S.
Materia(s) Ciencias
Abstract This work describes the development of a competitive flow-through FIA assay for digoxin using a molecularly imprinted polymer (MIP) as the recognition phase. In previous work, a number of non-covalent imprinted polymers were synthesised by “bulk” polymerisation. The digoxin binding and elution characteristics of these MIPs were then evaluated to obtain a highly selective material for integration into a sensor. The optimum MIP was synthesised by photo-initiated polymerisation of a mixture containing digoxin, MAA, EDGMA and AIBN in acetonitrile. The bulk polymer was ground and sieved and the template removed by Soxhlet extraction in MeOH/ACN. The MIP was packed into a flow cell and placed in a spectrofluorimeter to integrate the reaction and detection systems. The physical and chemical variables involved in digoxin determination by the sensor (nature and concentration of solution, flow rates, etc.) were optimised. Binding with the non-imprinted polymer (NIP) was also analysed. The new fluorosensor showed high selectivity and sensitivity, a detection limit of 1.7 × 10−2 μg l−1, and high reproducibility (R.S.D. of 1.03% and 1.77% for concentrations of 1.0 × 10−3 and 4.0 × 10−3 mg l−1, respectively). Selectivity was tested by determining the cross-reactivity of several compounds with structures analogous to digoxin. Under the assay conditions used, in which the potential interfering compounds were in concentrations 100 times higher than that of the analyte, no interference was recorded. The proposed fluorosensor was successfully used to determine digoxin concentration of human serum samples.
Palabras clave Digoxin
Molecular imprinting
Fluorosensor
Human serum analysis
Editor(es) Elsevier
Fecha 2008-06-15
Formato application/pdf
Identificador bibliuned:DptoCA-FCIE-Articulos-Gpaniagua-0008
http://e-spacio.uned.es/fez/view/bibliuned:DptoCA-FCIE-Articulos-Gpaniagua-0008
DOI - identifier https://doi.org/10.1016/j.bios.2008.01.018
ISSN - identifier 0956-5663 - eISSN 1873-4235
Nombre de la revista Biosensors and Bioelectronics
Número de Volumen 23
Número de Issue 11
Página inicial 1754
Página final 1758
Publicado en la Revista Biosensors and Bioelectronics. 2008. 23 (11), 1754-1758
Idioma eng
Versión de la publicación acceptedVersion
Tipo de recurso Article
Derechos de acceso y licencia http://creativecommons.org/licenses/by-nc-nd/4.0
info:eu-repo/semantics/openAccess
Tipo de acceso Acceso abierto
Notas adicionales The registered version of this article, first published in Biosensors and Bioelectronics, is available online at the publisher's website: Elsevier, https://doi.org/10.1016/j.bios.2008.01.018
Notas adicionales La versión registrada de este artículo, publicado por primera vez en Biosensors and Bioelectronics, está disponible en línea en el sitio web del editor: Elsevier, https://doi.org/10.1016/j.bios.2008.01.018

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Creado: Wed, 13 Mar 2024, 03:21:58 CET